Used in biacore x100, biacore 3000, biacore c, biacore 2000, biacore, biacore x, and biacore j. Page 94 the concentration of b is kept constant concavariable concentration of interactant a in the sample mixture the concentration of a is varied see section 11. Biacore spr technology cytiva, formerly ge healthcare life. Overview of biacore systems and their applications jason. This is based on the ability of the biomolecule of interest to interact with a specific binding partner, and may therefore be more informative than generic measurement techniques total protein concentration for example. Biacore t100 nanobiophysics usc dana and david dornsife.
The analyte is the interacting partner that is injected in solution over the sensor surface. This handbook describes biacore t100 control software and evaluation software, version 2. This edition of the biacore x100 handbook applies to systems running software version 2. Certificate grade cm5 chips are coated with capture antibody goat antimouse igg fc for mouse mab s, goat antihuman igg fc for chimeric, humanized or. Biacore t100 software version 2 university of oxford. In the case of classical injections singleanalyte injections, data sets were analyzed with biacore t100 evaluation software, biaevaluation 1. This video guides you through the sensorgram comparison evaluation workflow in the biacore t200 evaluation software. Ligand, analyte and capturing molecule in relation to the sensor surface. Biacore x100 handbook br100810 edition ac 9 important user information important user information biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures. Biacore 3000 complete system with biacore 3000 control software 3. The chip with two flow cells is being used and all the reagents are injected manually. The chips are equipped with four flow cells offering three ligands to be tested simultaneously first flow cell serves as a reference cell for nonspecific binding.
The handbook also includes a system description, safety instructions, specifications and a brief description of the spr principle. Sensor surface handbook br100571 biacore concentration analysis handbook br100512 used in biacore t200, biacore t100, biacore 4000 and biacore s51. Sep 17, 2009 ge healthcare introduces biacoretm x100 2. Optimizing multiple analyte injections in surface plasmon. Latest software updates and software notes software upgrade packages and additional licences software wizard templates to help speed up assay optimization and routine analyses handbooks covering system operationmaintenance and qflex kits system brochures and application notes. Software wizard templates to help speed up assay optimization and routine analyses. Ge biacore t200 getting started guide ligand analyte. Ge healthcare protein interaction analysis biacore sensor. The handbook, together with the associated reagent kit, will take you through the basic steps in a biacore experiment. Biacore t100 gxp software requires windows 2000 or windows xp professional and includes biacore t100 gxp control software and biacore t100 gxp evaluation software biacore t100 gxp handbook. Use this handbook together with the specific user documentation for your biacore system, which will describe how to implement the assay principles in the instrument and system software. Data file 29050011 aa labelfree interaction analysis biacore t200 software v2. August 28, 2015 uncategorized biacore, biacore t100, biacore t100 software, handbook, software, software handbook, t100, t100 software handbook wepholshasi. Biacore t100 gxp package ensures the highest level of gxp support and 21 cfr part 11 compliance.
The options that the ge biacore software provides are not useful, export curves just export a bitmap with a very bad quality by the way and export result table does not export the sensogram data. Biacore t200 software handbook, the instruction manual for biacore t200 control and evaluation software what to bring for your first experiment running buffer ligand molecule 2 50 gml in immobilization buffer, higher concentration if scouting conditions for immobilization. Surface plasmon resonance spr allows for the investigation of the functional nature of binding interactions and provides detailed kinetic information across a wide range of molecular weights. Br100648ae biacore t100 instrument software handbook web. Control software general features 5 okn leeeiacaaaccee r oko reeeaaieenae s. Biacore t100 instrument handbook guides you through operation and maintenance of the biacore t100 system. Biacore x ge healthcare is manual surface plasmon resonance based refractometer, appropriate for the introductory investigation of interactions with unknown properties. For the love of physics walter lewin may 16, 2011 duration. Currently, exported datasets from biacore surface plasmon resonance spr, fortebio biolayer interference bli, and biametrics single color reflectometry score can be uploaded and evaluated in anabel using 2 different evaluation methods. Biacore spr biosensors can be used for a wide variety of assays, including specificity, active concentration measurement, kinetics, and affinity and thermodynamic parameters. The software provides dedicated support for kinetics, affinity. Biaevaluation software handbook molecular interactions.
Course for new biacore t100 users introducing you to the biacore technology, biacore t100 system operation, assay development, data evaluation, and system maintenance so that you can start working with your own. Nov 22, 2016 this video guides you through the sensorgram comparison evaluation workflow in the biacore t200 evaluation software. Any functionality that is added in optional addon modules is described in separate documentation. Related manuals for ge healthcare biacore t100 laboratory equipment ge healthcare aktaprime plus operating instructions manual 66 pages compact liquid chromatography system designed for onestep purification of proteins at laboratory scale. Biacore sensorgram the systems also provide sensitive, accurate concentration measurements. Biacore t100 gxp software requires windows 2000 or windows xp professional and includes biacore t100 gxp control software and biacore t100 gxp evaluation software biacore t100 gxp handbook instrument and. Biacore t100 is a versatile, labelfree system for detailed studies of biomolecular interactions. This getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Biacore t200 instrument with reagent rack 2 and sample and reagent. Hitrap cartridge ge healthcare following the manufactures protocol. Data file 29050011 aa labelfree interaction analysis. Biacore epitope scouting analysis of antibodies are carried out on biacore 3000 for epitope mapping in order to identify mabs that recognize distinct separate epitopes on a single antigen.
Oct 12, 2015 biacore x100 software demo basic duration. We recommend to use biacore x for the complex interactions and initial screenings. Support for use in regu1ated gxp1 environments is provided in an optiona1 package that adds appropriate. It is fully automated and enables fast screening of many interactions.
Commonly, the analyte binds directly to the ligand, although. Data file 29050011 aa labelfree interaction analysis biacore. Sep 17, 2009 ge healthcare announces the launch of biacore x100 2. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Biaevaluation software handbook br100229 edition ah 5 introduction 1 1 introduction biaevaluation is a standalone software package for presentation and evaluation of sensorgram data from biacore analyses. Biacore t100 instrument handbook describes the instrumentation in the biacore t100 system, with instructions for operation, maintenance and troubleshooting. Sensorgram comparison evaluation workflow biacore t200. Biaevaluation software kit can be purchased without having to own a biacore system. Ge biacore t100 spr analyzer with computer software ebay. For multipleanalyte injections and optimizedexperiment analysis, inhouse software packages were developed with the matlab 7. Biacore spr technology cytiva, formerly ge healthcare. Biacore analysis service epitope mapping, kinetic analysis.
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